NGS Based Services

Better options, better outcomes

Robust analysis for the data insights you need. Complete sample-to-analysis solutions including customizable extraction and data analysis options available. We offer both standard and custom services for extraction, library preparation, sequencing, and bioinformatics.

Whole Genome Sequencing

NGS based Service

Whole Genome Sequencing
Whole Genome Sequencing, or WGS , is literally knowing all the ATGC content of a person’s DNA in the proper sequence. But knowing these is just the first part of the questionnaire. The tricky part is interpreting and analyzing all.
Sample requirement for WGS:

We accept gDNA, microbial cultures, plant tissues, mammalian tissues etc..
• gDNA sample: 8‐10µg of high molecular weight intact double stranded genomic DNA, 100 ‐ 200 µg of gDNA. gDNA samples will be subjected to quality check by gel electrophoresis & Nanodrop respectively.
• Microbial culture (non‐pathogenic): Pure isolated culture in broth or culture plate.
• Plant tissues: Minimum 3‐5 gm of specimen sample should be provided in ‐20⁰C.
• Mammalian tissue sample: 2 gm of tissue sample is required (aliquot 200mg in 10 vials).
 

Deliverables:

Dedicated  support at every step with real-time project updates.
Complete sequencing solutions from extraction to data interpretation.
Automated workflows increase scalability and improve reproducibility

Whole Transcriptome/ RNA Sequencing

NGS based Service

Whole Transcriptome Sequencing/RNA Sequencing
RNA sequencing (RNA-Seq) is a powerful method for studying the transcriptome qualitatively and quantitatively. It can identify the full archieve of transcripts, precisely defining the structure of genes, and thereby measuring the gene expression levels.
We provide you the best platform for your transcriptome sequence allows profiling of the whole population of mRNA and enables mapping and digital quantification of whole transcriptome.
Requirement:
We accept isolated RNA, microbial cultures, plant tissues etc.
• Isolated Total RNA: 8‐10µg of total RNA should be provided. RNA must not be degraded & should be free from DNA contamination.
Quality control of RNA samples: Samples will be subjected to both qualification, quantification and those having RIN> 6 will be QC passed. However,inclusion of low RIN value of the samples will be processed upon customer’s confirmation.
• Microbial cultures: Pure isolated culture in broth or culture plate.
• Plant tissues: Minimum 3‐5 gm of tissue sample should be transported in RNA later. The volume of RNA later should be at least ten mes the volume of tissue. Plant tissue samples should be harvested and immediately immersed in RNA later solution.

Metagenomics & Metatranscriptomics

NGS based Service

Metagenomics & Metatranscriptomics Services
Service types:
1. Metagenome diversity analysis using 16S/18S amplicon
2. Whole metagenome analysis
3. Whole metatranscriptome analysis
Sample requirement for Metagenomic/Metatranscriptomic Studies:
• Metagenomic DNA:
Purified 4‐5µg of intact double stranded gDNA with high molecular weight.
RNA contamination should be avoided.
• Metatranscriptomic RNA:
Total 8‐10 µg of RNA should be provided with RNA Integrity no (RIN)>6.
RNA contamination should be avoided.
Degradation of RNA should be kept on check.

Samll RNA Sequencing

NGS based Service

Small RNA sequencing
Small RNA sequencing enables analysis of miRNA and other small RNA species. Provides small RNA discovery and profiling.
Sample Requirement:
We accept total RNA, plant tissues, mammalian tissues etc.
• Isolated total RNA:
Total RNA
10‐20µg of total RNA should be provided with RNA Integrity Number (RIN) > 6.
Degradation of RNA should be kept on check.
Quality control of RNA samples:
Samples will be subjected to both qualification, quantification and those having RIN> 6 will be QC passed.

Exome Sequencing

NGS based Service

Exome Sequencing
Whole exome sequencing (WES) is a powerful tool to investigate genetic variations underlying cancers, Mendelian diseases, and complex human disorders. Targeting only protein coding regions, WES provides a more cost-effective approach than whole genome sequencing.
Delivarables:
• Data filtering and pre-processing
• Mapping reads on Human Reference
• Variant calling
• Functional annotation of variants
• Comprehensive compiled report and data